Fig. 4
Inhibition of Ca2+ influx channels and hyper-O-GlcNAcylation impairs MM dissemination in vivo. a–e Luciferase-labeled sgTRPM7 or WT RPMI8226 cells were injected into NSG mice via tail vein. A Representative bioluminescence imaging of mice taken at the time of inoculation (week 0) and weekly post-injection (week 1, 2, 3 and 4). b, c Quantification of whole-body luminescence signals normalized to their initial signal at week 0 (b) and relative to WT control cells (c). Data mean ± SD (n = 5). ***P < 0.001 versus WT cells; two-tailed Student’s t-test. d Representative ex vivo imaging from isolated internal organs including brain, lung, kidney, bone, heart, spleen, and liver at week 4 post-injection (see also Additional file 2: Fig. S15 for quantification of ex vivo IVIS images). e Representative IHC micrographs of femur bone and liver sections staining for human CD138 (see also Additional file 2: Fig. S17 for additional IHC micrographs). Scale bar = 100 μm. f–j Luciferase-labeled sgMGEA5 or WT RPMI8226 cells were similarly injected into NSG mice via tail vein and bioluminescence imaging and IHC staining were similarly performed as described in A–E (see also Additional file 2: Fig. S18 for additional IHC micrographs)