Fig. 6

Inhibition of ITGA4 and ITGB7 critically suppresses MM cell motility. a, b Human MM-derived RPMI8226 cells were genetically knocked down with sgRNA against ITGA4 and ITGB7 by CRISPR/Cas9. (left) Western blot analysis showing knockdown efficiency in sgITGA4 (a) and sgITGB7 (b) cells. c, d Cell migration and invasion were evaluated in sgITGA4 (c) and sgITGB7 (d) cells by Transwell assays at 48 h, where the penetrating cells were stained with Hoechst 33342 dye. (left) Representative micrographs of migrating/invading cells are shown. Scale bar = 100 μm. (right) Quantitative data showing the percentage of migrating/invading cells relative to WT control cells. Data mean ± SD (n = 4). ***P < 0.001 versus WT; two-tailed Student’s t-test