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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: O-GlcNAcylation homeostasis controlled by calcium influx channels regulates multiple myeloma dissemination

Fig. 7

ITGA4 and ITGB7 are favorable downstream targets of Ca2+ influx/O-GlcNAc-mediated MM cell motility. a–c Rescue experiments were performed in sgTRPM7 (a), sgORAI1 (b), and sgSTIM1 (c) RPMI8226 cells by transfection of the cells with ITGA4, ITGB7 or control pcDNA plasmid. ITGA4 (upper) and ITGB7 (lower) levels were evaluated by Western blot analysis at 48 h post-transfection prior to experiments and representative blots were shown. d–f Cell migration and invasion in ITGA4 or ITGB7-overexpressing sgTRPM7 (d), sgORAI1 (e), and sgSTIM1 (f) cells by Transwell assays at 48 h, where the penetrating cells were stained with Hoechst 33342 dye. Quantitative data showing the percentage of migrating/invading cells relative to transfected control cells. Data mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 versus pcDNA-transfected control cells; one-way ANOVA with Tukey’s multiple comparison test. #P < 0.05, ##P < 0.01, ###P < 0.001 versus pcDNA-transfected sgTRPM7, sgORAI1, or sgSTIM1 cells; one-way ANOVA with Tukey’s multiple comparison test. ns, not significant

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