Fig. 3

Overexpressed WDR3 promoted the aggressive behavior of pancreatic cancer cells in vitro and pancreatic tumor growth in vivo. a and b RT-PCR (a) and western blot analyses (b) of WDR3 expression in PANC-1, MIA PaCa-2, and BxPC-3 cells infected with pcDNA3.1 or WDR3 plasmid. GAPDH served as an internal reference. Data are presented as the mean ± SD of three independent experiments. WDR3 group was compared with pcDNA3.1 group. Statistical analyses were performed with one-way ANOVA followed by Tukey’s multiple comparison’s tests. ***, P < 0.001. C-E. PANC-1, MIA PaCa-2, and BxPC-3 cells were infected with pcDNA3.1 or WDR3 plasmid. The cells were harvested for colony formation (c), MTS (d), and Transwell invasion assays (e) after 48 h of culture. Each bar represents the mean ± SD of three independent experiments. **, P < 0.01; ***, P < 0.001. F-H. PANC-1 cells infected with pcDNA3.1 or WDR3 plasmid were subcutaneously injected into nude mice. The tumors were harvested and photographed (f) on day 21. Data for tumor volume (g) and tumor mass (h) are shown as the mean ± SD (n = 5). WDR3 group was compared with pcDNA3.1 group. Statistical analyses were performed with one-way ANOVA followed by Tukey’s multiple comparison’s tests. ***, P < 0.001