Fig. 5

WDR3 knockdown enhanced the anti-pancreatic cancer effect of YAP1 inhibition. a RT-PCR analysis was used to detect the mRNA expression levels of CTGF and CYR61 in pancreatic cancer cells infected with sh-Control or sh-WDR3 #1 and treated with or without TED-347. GAPDH served as an internal reference. Data are shown as the mean ± SD (n = 3). Statistical analyses were performed with one-way ANOVA followed by Tukey’s multiple comparison’s tests. ***, P < 0.001. b-c. PANC-1 cells infected with sh-Control or sh-WDR3 #1 and treated with or without TED-347 were harvested for MTS (b) and colony formation assays (c). Data are shown as the mean ± SD (n = 3). Statistical analyses were performed with one-way ANOVA followed by Tukey’s multiple comparison’s tests. ***, P < 0.001. d-f. PANC-1 cells infected with sh-Control or sh-WDR3 #1 were subcutaneously injected into nude mice. The mice were treated with TED-347 3 times on days 1, 4, and 7 at a dose of 20 mg/kg. The tumors were harvested and photographed (d) on day 21. Data for tumor volume (e) and tumor mass (f) are shown as the mean ± SD (n = 5). Statistical analyses were performed with two-way ANOVA followed by Sidak’s multiple comparison’s tests. ***, P < 0.001