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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Overexpressed WDR3 induces the activation of Hippo pathway by interacting with GATA4 in pancreatic cancer

Fig. 7

GATA4, acting as a transcription factor, transcriptionally upregulated YAP1 expression in pancreatic cancer cells. a-b RT-PCR analysis (a) and western blot analysis (b) were used to detect the mRNA and protein expression levels of YAP1 in pancreatic cancer cells infected with sh-Control or sh-GATA4s. GAPDH served as an internal reference. Data are shown as the mean ± SD (n = 3). Statistical analyses were performed with two-way ANOVA followed by Sidak’s multiple comparison’s tests. ***, P < 0.001. C-D. RT-PCR analysis (c) and western blot analysis (d) were used to detect the mRNA and protein expression levels of YAP1 in pancreatic cancer cells infected with pcDNA3.1 or GATA4.GAPDH served as an internal reference. Data are shown as the mean ± SD (n = 3). Statistical analyses were performed with two-way ANOVA followed by Sidak’s multiple comparison’s tests. ***, P < 0.001. e The Eukaryotic Promoter Database was searched to evaluate potential YAP1 promoter binding by GATA4 (− 649 bp, − 467 bp, and 66 bp), and the ChIP primer sequences (Table S3) were designed for the gene locus from − 700 bp to − 400 bp. f GATA4 ChIP-qPCR of YAP1 in PANC-1, MIA PaCa-2, and BxPC-3 cells were performed. All data are shown as the mean ± SD of three replicates. Statistical analyses were performed with two-way ANOVA followed by Sidak’s multiple comparison’s tests. ***, P < 0.001. G-H. GATA4 ChIP-qPCR of YAP1 in PANC-1, MIA PaCa-2, and BxPC-3 cells with WDR3 silenced (g) or overexpressed (h). was performed. All data are shown as the mean ± SD of three replicates. Statistical analyses were performed with two-way ANOVA followed by Sidak’s multiple comparison’s tests. ***, P < 0.001

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