Fig. 1

HBP1 regulates AFP expression. a There is a negative correlation between the expression of HBP1 and AFP in hepatoma tissues. The gene expression data were derived from the primary tumors who received surgery or TACE and then monitored for relapse for up 10 years. The protein levels of HBP1 and AFP were measured by IHC in hepatoma tissues. Scale bar, 100 μm. b Elevated expression of HBP1 in tumor is associated with higher survival rate of hepatoma patients. The overall survival curves were performed in two groups of hepatoma patients with high or low level of HBP1. The protein levels of HBP1 were analyzed based on tumor TNM classification. c There is a negative correlation between the expression of HBP1 and HBV infection. The protein level of HBP1 was measured by IHC in hepatoma tissues. d HBP1 overexpression decreases AFP protein and mRNA expression. The protein levels of HBP1 and AFP in cell lysate and supernatant were measured by Western blotting in HepG2 and PLC5 cells transfected with pCDNA3-HBP1 or pCDNA3 (as a control). GAPDH and GST were used as a loading control respectively (left panel). The mRNA level of AFP was measured by Realtime-PCR in HepG2 and PLC5 cells transfected with pCDNA3-HBP1 or pCDNA3 (right panel). e HBP1 knockdown by shRNA increases AFP protein and mRNA expression. The protein levels of HBP1 and AFP in cell lysate and supernatant were measured by Western blotting in HepG2 and PLC5 cells stably transfected with pLL3.7-shHBP1 or pLL3.7 (as a control) through lentiviral infection. GAPDH and GST were used as a loading control respectively (left panel). The mRNA level of AFP was measured by Realtime-PCR in HepG2 and PLC5 cells stably transfected with pLL3.7-shHBP1 or pLL3.7 (right panel) through lentiviral infection. Error bars represent S.D. *, p < 0.05, **, p < 0.01