Fig. 8

HBP1 deletion aggravates DEN/CCl₄-induced hepatoma. a Representative images of hepatoma sections from 20 weeks after DEN/CCl₄ treatment in wild type or HBP1^−/− mice. b The protein levels of HBP1 and AFP in the hepatoma described in Fig. 8a were measured by Western blotting. β-actin was used as a loading control. c The mRNA level of AFP was measured by Realtime-PCR in the hepatoma described in Fig. 8a. d Serum AFP level in the mice described in Fig. 8a. e The IHC analyses of Ki67 in the mice described in Fig. 8a. f Model of the role of HBP1 in inhibiting the occurrence and development of hepatoma by repressing the expression of AFP. Scale bar, 100 μm. Bar graphs show numbers of Ki67-positive cells per 100 × field. Error bars represent S.D. *, p < 0.05, **, p < 0.01