Fig. 2

ZNF561-AS1 acts as a ceRNA for miR-26a-5p and miR-128-3p in CRC cells. a Schematic of potential miR-26a-5p and miR-128-3p binding sites on ZNF561-AS1. b Both miR-26a-5p and miR-128-3p were decreased in HCT-116 cells. n = 3, *p < 0.01. c ZNF561-AS1 silencing elevated miR-26a-5p and miR-128-3p levels in HCT-116 cells. n = 3, *p < 0.05. d ZNF561-AS1 overexpression suppressed miR-26a-5p and miR-128-3p levels in HCT-116 cells. n = 3, *p < 0.05. e Transfection of miR-26a-5p or/and miR-128-3p mimics suppressed ZNF561-AS1 expression. n = 3, *p < 0.05. f Relative enrichments of ZNF561-AS1, miR-26a-5p, as well as miR-128-3p in RISC was examined by RIP assay using anti-Ago2 antibody. n = 3, *p < 0.01. g Luciferase reporter assay. HCT-116 cells were transfected as indicated. Relative luciferase activity was assessed 48 h post transfection. n = 3, *p < 0.01