Fig. 5

RSF6 silencing repressed cell proliferation and triggered apoptosis in CRC cells. a Knockdown of SRSF6 inhibited HCT-116 cells growth. n = 3, *p < 0.01. b Colony formation assay. HCT-116 cells transfected with SRSF6 siRNA or control siRNA were seeded in 6 cm dishes for 12 days culture. Formed colonies were stained with crystal violet and counted. n = 3, *p < 0.05. c Proliferation capabilities of ZNF561-AS1 siRNA or control siRNA transfected HCT-116 cells was examined by Ki-67 immunofluorescence staining. n = 3. d Knockdown of SRSF6 induced cell apoptosis assessed by AO/EB staining. n = 3, *p < 0.01. e Knockdown of SRSF6 induced cell apoptosis assessed by TUNLE staining. n = 3