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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Oncogenic lncRNA ZNF561-AS1 is essential for colorectal cancer proliferation and survival through regulation of miR-26a-3p/miR-128-5p-SRSF6 axis

Fig. 6

Exogenous SRSF6 expression rescued proliferation of ZNF561-AS1-depleted CRC cells in vitro and in vivo. HCT-116 cells were co-transfected as indicated. a RT-qPCR analysis of ZNF561-AS1, miR-26a-5p, and miR-128-3p levels. n = 3, *p < 0.05. b SRSF6 expression was examined by immunoblotting. Actin served as loading control. Representative blots were shown (upper penal). Ratios of level of SRSF6 vs Actin were calculated after densitometric analysis of blot images using NIH Image J 1.61 (lower panel). Data were from three individual experiments. *p < 0.01. c Enforced expression of SRSF6 restored cell growth capabilities in HCT-116 cells depleted of ZNF561-AS1. n = 3, *p < 0.05. d Enforced expression of SRSF6 restored Ki-67 expression in HCT-116 cells depleted of ZNF561-AS1. n = 3. e ZNF561-AS1 expression in xenograft tumors. n = 3, *p < 0.01. f Co-transfection of SRSF6 expression vector restored SRSF6 level in xenograft tumors. Actin served as loading control. n = 3, *p < 0.01. g Exogenous expression of SRSF6 rescued tumor volume. n = 3, *p < 0.05. h Exogenous expression of SRSF6 rescued tumor weight. n = 3, *p < 0.05. i SRSF6 overexpression restored Ki-67 levels in xenograft tumors. n = 3

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