Fig. 4From: TP53 drives abscopal effect by secretion of senescence-associated molecular signals in non-small cell lung cancerCell irradiation induces the expression of DNA:RNA hybrid structures in NSCLC cell lines. a Immunofluorescence staining with S9.6 antibody. The images are representative of A549, A549sh/p53 and H1299 cells exposed to 10 or 20 Gy. Scale bars are 10 μm. b Regional foci intensity quantification. Effect of increasing irradiation doses on grand median intensities of nuclear and cytoplasmic foci of DNA:RNA hybrids. Data are presented as grand median ± MAD. c Dot blot analysis of RNA extracted from exosome or microvesicle fraction 72 h post-irradiation and immunoblotted with S9.6 antibody. (*p < 0.05, **p < 0.01 ***p < 0.001)Back to article page