Fig. 1

CPSF6 is upregulated and predicts poor prognosis in HCC. a IHC staining of CPSF6 in surrounding non-tumor and HCC tissues. Percentage of CPSF6 IHC was shown in bar graph. Scale bar, 50 μm. b Western blot assay for detecting CPSF6 expression in 16 paired surrounding non-tumor (N) and HCC tumor (T) tissues. β-actin was used as internal control. c qRT-PCR analysis of CPSF6 mRNA in 36 paired surrounding non-tumor (N) and HCC tumor (T) tissues. GAPDH was used as internal control. d, e Kaplan–Meier analysis of the correlation of CPSF6 protein expression with OS (d) and DFS (e). The expression level of CPSF6 protein was detected by immunohistochemistry. f qRT-PCR analysis of CPSF6 mRNA in TCGA liver cancer samples. g,h Kaplan–Meier analysis of OS (g) and DFS (h) data from TCGA liver cancer data containing 364 patients. The data of g and f can be obtained through online website (http://kmplot.com/analysis/).**p < 0.001, Student’s t-test