Fig. 2

CPSF6 accelerates the growth of HCC cells in vitro and in vivo. a The protein level of CPSF6 in different cell lines was assessed by western blotting. β-actin was used as internal control. b The protein level of CPSF6 in the CPSF6-overexpressing (CPSF6-OE) HL-7702 was assessed by western blotting. β-actin was used as internal control. c CPSF6 knockdown efficiency in Huh-7 with two CPSF6 specific shRNAs (shCPSF6–1 and shCPSF6–2) was examined by western blotting. β-actin was used as internal control. d Proliferation curves of the CPSF6-overexpressing HL-7702 cells and the control (Ctrl) were shown. e Proliferation curves of the CPSF6 knockdown Huh-7 cells and the control (shCtrl) were shown. f The cell viability of HL-7702 cells with CPSF6 overexpression and the control was examined by MTT assay. g The cell viability of CPSF6 knockdown Huh-7 cells and Huh-7 cells (the control) was examined by MTT assay. h,i Colony formation analysis of the indicated cell lines. j HL-7702 xenograft tumor growth with or without overexpression of CPSF6. k The weight of HL-7702 tumors at the end point was shown. l Huh-7 xenograft tumor growth with or without CPSF6 knockdown. m The weight of Huh-7 tumors at the end point was shown. n, o IHC analysis of CPSF6 and Ki67 expression in tumors. Scale bars, 100 μm. **p < 0.001, Student’s t test