Fig. 7

CPSF6 upregulates glycolysis and promotes aerobic glycolysis through NQO1. a Glucose uptake in control and NQO1-overexpressing HL-7702 cells. b Cellular lactate levels in control and NQO1-overexpressing HL-7702 cells. OCR (c) and ECAR (d) of control and NQO1- overexpressing HL-7702 cells measured by the Seahorse Bioscience XF96 analyzer. e Glucose uptake in control and CPSF6-overexpressing HL-7702 cells with or without NQO1 knockdown. f Cellular lactate levels in control and CPSF6-overexpressing HL-7702 cells with or without NQO1 knockdown. OCR (g) and ECAR (h) of control and CPSF6-overexpressing HL-7702 cells with or without NQO1 knockdown measured by the Seahorse Bioscience XF96 analyzer. i Glucose uptake in control and CPSF6 knockdown Huh-7 cells with or without overexpression of NQO1. j Cellular lactate levels in control and CPSF6 knockdown Huh-7 cells with or without overexpression of NQO1. OCR (k) and ECAR (l) of control and CPSF6 knockdown Huh-7 cells with or without overexpression of NQO1 measured by the Seahorse Bioscience XF96 analyzer. **p < 0.001, Student’s t test