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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Selinexor versus doxorubicin in dedifferentiated liposarcoma PDXs: evidence of greater activity and apoptotic response dependent on p53 nuclear accumulation and survivin down‐regulation

Fig. 2

Characterization of patient-derived DDLPS models. a, Representative pictures of PDX models and corresponding clinical tumors. The histology was assessed on hematoxylin and eosin (H&E)-stained slides. MDM2 amplification was detected at the protein level by MDM2 immunostaing. b, FISH analysis: Spectrum Orange labeled Chromosome 12 centromere and Spectrum Green labeled MDM2. High level of MDM2 amplification with clustering of gene copies are observed in both patient tissues and PDXs. c, Quantification of MDM2, CDK4 and HMGA2 copy number variation in PDXs by ddPCR. d, Assessment of MDM2, CDK4 and HMGA2 protein expression in cell lines by western blotting. A representative blot of three independent experiments is shown. For each protein, band intensity was quantified using Image J normalized to loading control reported below

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