Fig. 4

FGF19-FGFR4 signaling axis switches the role of MT in cell motility upon long-term exposure to high-dose MT. a Increased levels of ATF4, FGF19, and Vimentin in MT-SE and MT-LE HN30 cells. b The increased ATF4 amount on the FGF19 gene promoter in MT-LE HN30 cells than MT-SE HN30 cells determined by ChIP-qPCR. c The morphological changes between MT-LE and its parental HN30 cells photographed under an inverted phase-contrast microscopy. d Comparison of migration capacity between MT-LE and its parental HN30 cells by wound healing assays. e Comparison of ex vivo invasion potential between MT-LE and its parental HN30 cells by SeedEZ™ 3D rings. f, g The effect of FGF19 knockdown (shFGF19) on cell migration (f) and Vimentin protein levels (g) in MT-LE HN30 cells. h, i The effect of H3B-6527 on cell migration (h) and Vimentin protein levels (i) in MT-LE HN30 cells. In (d, e, f, h) representative images and quantitative data from three independent experiments are shown in the left and right panels, respectively. MT-SE, HN30 cells under 12-h exposure of MT; MT-LE, HN30 cells under long-term exposure of MT. *p < 0.05; **p < 0.01