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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: RETRACTED ARTICLE: Downregulation of lncRNA ZNF582-AS1 due to DNA hypermethylation promotes clear cell renal cell carcinoma growth and metastasis by regulating the N(6)-methyladenosine modification of MT-RNR1

Fig. 5

ZNF582-AS1 overexpression decreased rRNA adenine N(6)-methyltransferase A8K0B9 expression. a The overexpression efficiency of ZNF582-AS1 in OSRC2 cells. b iTRAQ results showed that 69 proteins were downregulated and 75 proteins were upregulated in ZNF582-AS1-overexpressed OSRC2 cells compared with control OSRC2 cells. c The expression of these 144 differently expressed proteins in ZNF582-AS1-overexpressed OSRC2 cells and control cells. d Biological Process GO term enrichment analysis of the complete 144 statistically significant proteins. e and f Validation of the presence of A8K0B9 protein. g A8K0B9 expression was decreased in ZNF582-AS1-overexpressed OSRC2 cells compared with control cells. h Changes of A8K0B9 protein level in OSRC2 cells after transient transfection of different doses of ZNF582-AS1 overexpression plasmid. i Estimation of the binding propensity of A8K0B9-ZNF582-AS1 pair. j RNA pull-down assay indicated that A8K0B9 protein bound specifically to ZNF582-AS1

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