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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: HMGA1-TRIP13 axis promotes stemness and epithelial mesenchymal transition of perihilar cholangiocarcinoma in a positive feedback loop dependent on c-Myc

Fig. 5

FBXW7 suppressed TRIP13-induced stemness and EMT. a TRIP13 knockdown decreased FBXW7 expression in QBC-939 and FRH-0201 cells. b Luciferase assays showed that TRIP3 inhibited FBXW7 transcription in QBC-939 and FRH-0201. c Silencing TRIP13 suppressed pCCA proliferation and knocking down FBXW7 had contrary effects. d,e Transwell assay showed that silencing FBXW7 promoted the migration(d) and invasion(e) which was decreased by TRIP13 knockdown. f,g QBC-939 and FRH-0201 were transfected with shTRIP13 and/or siFBXW7. The biomarkers of stemness (CD44 and OCT4)(f) and 7-day sphere formation assay(g) were performed. (H) EMT biomarkers were detected after regulating TRIP13 and/or FBXW7 expression in QBC939(left) or FRH0201(right). In (b,d,e,g), ** and *** represents P < 0.01 and < 0.001, compared with corresponding control group or indicated groups. In (b,d), the P value was calculated with T-test(b,d,g) or one-way ANOVA(c). Analyzed data were from three independent experiments, and each subgroup was performed at least in triplicate

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