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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: HMGA1-TRIP13 axis promotes stemness and epithelial mesenchymal transition of perihilar cholangiocarcinoma in a positive feedback loop dependent on c-Myc

Fig. 7

Inhibitors of HMGA1, TRIP13 or c-Myc can block their feedback and suppress pCCA progression. a In the presence of Wnt3A(100 ng/ml), 10 μM 10,058-F4, 10μg/ml HMGA1 inhibitor Netropsin and 10 μM TRIP13 inhibitor DCZ0415 were used to incubate QBC-939 for 12 h. All these inhibitors inhibited the expression of c-Myc, TRIP13 and HMGA1. b,c WB b and sphere formation(c) indicated the inhibitors of c-Myc, HMGA1 and TRIP13 decreased stemness of pCCA. d Inhibitors of c-Myc, HMGA1 and TRIP13 attenuated EMT of pCCA. e,f Inhibitors of c-Myc, HMGA1 and TRIP13 attenuated migration (e) and invasion (f) of pCCA in Wnt3a stimulation. ** and *** represents P < 0.01 and < 0.001, calculated with T-test. Analyzed data were from three independent experiments and displayed by the mean + S.E.M.

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