Fig. 2

Biological evaluation of activated pathways in PDAC cell lines identifies FAK. a. ssGSEA of phosphorylated proteins highlight focal adhesion pathway in all cell lines. Heatmap shows enrichment score (ES) of top 20 up and downregulated KEGG pathways (coloring based on ES, red = upregulated, blue = downregulated). b. Western blot of FAK expression and phosphorylation of tyrosine-sites validates phosphoproteome analysis (loading control ACTB). c. Western blot of FAK in primary cell line panels confirms abundant expression in other PDAC models (loading control ACTB). d. Kinase-substrate relations of FAK are visualized in an interactive network of cell line Suit-2, which represents the highest relative FAK phosphorylation (visualization by Phosphopath [22])