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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Circ-MBOAT2 knockdown represses tumor progression and glutamine catabolism by miR-433-3p/GOT1 axis in pancreatic cancer

Fig. 2

Circ-MBOAT2 absence restrained the process of pancreatic cancer. a The impact of circ-MBOAT2 knockdown on the expression of circ-MBOAT2 was determined by qRT-PCR in PANC-1 and SW1990 cells with β-actin as an internal reference. b-d MTT and cell colony formation assays were employed to reveal the impact of circ-MBOAT2 silencing on the proliferation of PANC-1 and SW1990 cells. e Flow cytometry analysis was performed to illustrate the impact of circ-MBOAT2 knockdown on the apoptosis of PANC-1 and SW1990 cells. f and g The impacts of circ-MBOAT2 downregulation on the invasion and migration of PANC-1 and SW1990 cells were demonstrated by transwell invasion and wound-healing assays, respectively. h-j Glutamine, α-KG and glutamate assay kits were used to detect glutamine consumption, α-KG production and glutamate production, respectively, in PANC-1 and SW1990 cells transfected with si-circ-MBOAT2 or si-NC. **P < 0.01, ***P < 0.001 and ****P < 0.0001

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