Fig. 6

MiR-433-3p targeted GOT1 in PANC-1 and SW1990 cells. a The putative binding sites between miR-433-3p and GOT1 were predicted by starbase online database. b and c Dual-luciferase reporter assay was carried out to demonstrate the associated relationship between miR-433-3p and GOT1. d QRT-PCR was performed to detect GOT1 mRNA expression in 34 pairs of pancreatic cancer and adjacent normal pancreatic tissues. e UALCAN dataset was used to predict GOT1 expression in pancreatic cancer tissues (N = 179) and paracancerous normal tissues (N = 171). f and g Western blot analysis was performed to detect GOT1 protein expression in 23 pairs of pancreatic cancer and matched normal tissues as well as HPDE, PANC-1 and SW1990 cells. h Spearman correlation analysis was carried out to illustrate the linear relationship between GOT1 expression and miR-433-3p expression. i The impacts of miR-433-3p mimics and inhibitors on GOT1 protein expression were determined by western blot analysis. The U6 and β-actin were employed for the normalization of miR-433-3p and GOT1, respectively. *P < 0.05, ***P < 0.001 and ****P < 0.0001