Fig. 5

CTD affects the intracellular localization of MDM2 and p53 and p53 activity. a Effects of CTD on the intranuclear content of p53 and MDM2. The cells were stained for p53 (red) and MDM2 (Green), and the nuclei were counterstained with DAPI (blue). The localization of p53 and MDM2 expressions in the cells is indicated in the merged image. Scale bar = 200 μm. b p53 was upregulated, and MDM2 was suppressed and translocated to the nucleus following the CTD treatment. The endogenous p53 and MDM2 were detected in the cytoplasm and nucleus of CRC cells treated with CTD for 24 h using an anti-p53, MDM2, tubulin, and lamin A/C antibodies for western blotting