Fig. 7

CTD inhibits colon cancer cell growth irrespective of cellular p53 status. a The expression of p53 in these two cell lines by western blot. b The growth of HCT-116 p53+/+ and p53−/− cells were determined by the MTT assay after treatment with various CTD concentrations (0, 2.5, 5, or 10 μM) for 24, 48, 72, or 96 h, and similar results were obtained. c The effect of treatment with CTD on HCT-116 p53+/+ and p53−/− cells was evaluated. d The effect of CTD on apoptosis was assessed in HCT-116 p53+/+ and p53−/− cells. All the data are shown as means ± SD of values from triplicate samples. (*p < 0.05, **p < 0.01, *** p < 0.001) indicate a significant difference compared to the control