Fig. 3

CircFAM73A enhances the stem cell-like property in GC cells. a Representative images of formatted spheres among indicated cells. Scale bar: 100 μm. b Sphere formation abilities were accessed by the number of spheres. c Effects of circFAM73A alteration on capacity of sphere formation were measured by extreme limiting dilution assay. d Representative flow cytometric histograms and quantification of the CD44 positive proportion among indicated cells. e Several stemness-related factors including CD44, SOX-2, OCT-4 and Nanog were measured by qRT-PCR. Pseudocolors represent the intensity scale of expression in circFAM73A vs. vector cells and si-circFAM73A vs. control cells calculated by log2 transformation. f Western blot of stemness-related factors including CD44, SOX-2, OCT-4, and Nanog among indicated cells. Graph represents mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001