Fig. 5

CircFAM73A regulates HMGA2 expression by miR-490-3p. a Correlation between circFAM73A and HMGA2 according to our GC samples. b Western blot of HMGA2 was detected in BGC823 transfected with indicated vectors. c Representative flow cytometric histograms and quantification of the CD44 positive proportion in BGC823 transfected with indicated vectors. d Western blot of stemness-related factors including CD44, SOX-2, OCT-4, and Nanog in BGC823 transfected with indicated vectors. e FISH assay of circFAM73A and miR-490-3p in 15 paired GC and adjacent tissues from patients. FISH scores were further assessed. Nuclei were stained with DAPI. Scale bar: 50 μm. f IHC staining of HMGA2 in in 15 paired GC and adjacent tissues. Scale bar: 100 μm. g The IHC scores of HMGA2 were further quantified. h Three-dimensional scatter plot of circFAM73A, miR-490-3p and HMGA2 15 paired GC and adjacent tissues. Graph represents mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001