Fig. 7

CircFAM73A regulates HMGA2 expression level to promote GC growth and metastasis in vivo. a The images of xenograft tumors of sacrificed mice subcutaneous injected with indicated cells 4 weeks after injections. b, c Growth curves of xenograft tumors. Tumor volume was calculated by 1/2 (length × width2). d Representative images of bioluminescent images of mice by IVIS Imaging system and HE staining of lung tissues. Scale bar: 200 μm. e Quantification of metastatic foci in mice lungs injected with indicated cells. g, h Immunohistochemistry staining and IHC scores of HMGA2 and CD44 in respective xenograft tumor tissues. * vs the group of first column, ^# vs the group of second column. i Representative images of HE staining of liver tissues from mice with portal veins injection. Scale bar: 100 μm. j Liver indexes (liver weight/ body weight) of each group were calculated. Graph represents mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001, ^#p < 0.05, ^{# #}p < 0.01, ^{# # #}p < 0.001