Fig. 6

HMGB1 mediates the promoting effect of YAP on glioma growth. a Representative images of EdU assay of the vector and YAP over-expression cells with or without HMGB1 down-regulation. Scale bar: 100 μm. b Quantification results of (a). c The effect of HMGB1 knockdown on cell proliferation was detected by CCK-8 assay in the vector and YAP over-expression cells. d Schematic illustration of the in vivo experimental workflow. e Representative pseudocolor bioluminescence images of each group at indicated day after implantation. f Quantitative analysis of the photon flux. g Overall survival was determined by Kaplan-Meier survival curves and a log-rank test was used to assess the statistical significance of the differences (n ≥ 7). All data are expressed as the mean ± SD of values from experiments performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001