Fig. 2

IGFBP3 enhanced IR-induced cell-killing in vivo. a Upper: The schematic for orthotopic xenograft with radiation (8 Gy IR) treatment and sacrifice 3–4 weeks after IR. Lower: Tumor weight (left panel) and volume (right panel) are shown for mice (n = 7–8) orthotopically injected with OEC-M1 cells with ectopic IGFBP3 expression (OEC-M1 PB-IGFBP3) or vector control (OEC-M1 PB) cells, with or without subsequent treatment with 8 Gy IR, and sacrificed 3–4 weeks after irradiation. b Left: Representative images of Ki-67-labeled OEC-M1 PB and PB-IGFBP3 tumor sections from mice treated with 8 Gy IR (200× magnification). Right: The mean percentage of Ki-67-positive cells per field. c Upper: The schematics for orthotopic xenograft with radiation (8 Gy IR) treatment and sacrifice 72 h after irradiation. Lower: Tumor weight (left panel) and volume (right panel) were shown for mice (n = 5) orthotopically injected with OEC-M1 PB or OEC-M1 PB-IGFBP3 cells, with subsequent treatment with 8 Gy IR, and sacrificed 72 h after irradiation. d Left: Representative images of Ki-67 and (e) TUNEL-labeled OEC-M1 PB and PB-IGFBP3 tumor sections from mice treated with 8 Gy IR and sacrificed 72 h after irradiation (200× magnification). Right: The mean percentage of Ki-67-positive and (e) TUNEL-positive cells per field. Black arrows indicate apoptotic cells. Results from one of at least two independent experiments are shown. Values are expressed in mean ± SE. **p < 0.01; ***p < 0.001