Fig. 3

SPOCD1-AS induces the MMT process of mesothelial cells. a RNA-FISH analysis of SPOCD1-AS with Cy3-labeled probes (red). 18S and U6 served as the cytoplasm and nucleus control, respectively. Scale bar, 20 μm. b qRT-PCR analysis of SPOCD1-AS expressions in cytoplasm and nucleus of MeT-5A cells. GAPDH and β-actin served as the cytoplasm control and U1 as the nucleus control. c-f MeT-5A cells were transduced with SPOCD1-AS overexpression and control lentivirus. After transduction, morphology alteration was assessed (c), MMT-related proteins were detected by western blot analysis (d), migration assay (e) and adhesion assay (f) were performed. Scale bar, 100 μm. g-i MeT-5A cells were transduced with two SPOCD1-AS shRNAs and negative control. After transduction, MMT-related proteins were detected by western blot analysis (g), migration and adhesion assays were performed, scale bar, 100 μm (h, i). Data are representative of at least three independent experiments and are presented as mean ± SD. *p < 0.05, **p < 0.01