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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: LncRNA SPOCD1-AS from ovarian cancer extracellular vesicles remodels mesothelial cells to promote peritoneal metastasis via interacting with G3BP1

Fig. 4

EVs carrying SPOCD1-AS induce the MMT process in mesothelial cells. a EVs from control IOSE-80 cells (IOSE-80NC EVs) and IOSE-80 cells with SPOCD1-AS overexpression (IOSE-80OE EVs) were purified. SPOCD1-AS in EVs was detected by qRT-PCR, and PCR product was showed by electrophoresis. b-e MeT-5A cells were fed with IOSE-80NC EVs and IOSE-80OE EVs, PBS as control. SPOCD1-AS in MeT-5A cells was detected by qRT-PCR (b), MMT-related proteins were detected by western blot analysis (c), migration and adhesion assays were performed. Scale bar, 100 μm (d, e). f EVs from control A2780 cells (A2780NC EVs) and A2780 cells with SPOCD1-AS stable knockdown (A2780KD EVs) were purified. SPOCD1-AS in EVs was detected by qRT-PCR, and PCR product was showed by electrophoresis. g-j MeT-5A cells were fed with A2780NC EVs, A2780KD EVs, PBS as control. SPOCD1-AS in MeT-5A cells was detected by qRT-PCR (g), MMT-related proteins were detected by western blot analysis (h), migration and adhesion assays were performed. Scale bar, 100 μm (i, j). Data are representative of at least three independent experiments and are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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