Fig. 5
EVs carrying SPOCD1-AS promote ovarian cancer peritoneal metastasis in vivo. a Schematic protocol for establishment of orthotopic ovarian cancer mouse model by injecting SKOV3-luc cells (1 × 10^6) into the left ovarian subepithelium. b Representative images of orthotopic mouse models at time of killing. The left image indicates the IVIS imaging. The middle image shows dissected primary tumour. The right image showes the metastatic tumours in the peritoneal cavity after the primary tumour was dissected from mice. c IOSE-80NC EVs, IOSE-80OE EVs, A2780NC EVs, A2780KD EVs (20 μg per 100 μl for every mouse) and PBS control (100 μl) were intraperitoneally injected every other day from week 1 for 10 times (n = 5 for every group). The mice were sacrificed by intravenous injection of pentobarbital (100 mg/kg) at week 6. Bioluminescence images of the dissected primary tumor and the peritoneal metastatic tumors at the time of killing. Photon count was calculated and showed as median with interquartile range. *p < 0.05, **p < 0.01. d GW4869 (200 μl of 0.1 mg/ml GW4869 solution, total 20 μg) or 1.25% DMSO saline (200 μl) per mouse were injected intraperitoneal twice a week from week 1 for 8 times (n = 6 for each group). Then the mice were sacrificed at week 6. Bioluminescence images of the dissected primary tumor and the peritoneal metastatic tumors at the time of killing. Photon count was calculated and showed as median with interquartile range. **p < 0.01. e Histological features of primary and metastatic tumors and peritoneum in mouse model. Hematoxylin and eosin (H&E) staining. Scale bar, 200 μm and 100 μm