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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: LncRNA SPOCD1-AS from ovarian cancer extracellular vesicles remodels mesothelial cells to promote peritoneal metastasis via interacting with G3BP1

Fig. 7

G3BP1 interfering peptide suppresses the MMT process by blocking SPOCD1-AS/G3BP1 interaction. a The schematic domain structure of G3BP1. b RRM domain truncated and mutant F380L/F382L G3BP1 (marked as ΔRRM and Mut) were detected in RNA pull-down followed with western blot analysis. Empty vector and full-length G3BP1 (marked as Vec and WT) were as controls. c The schematic structure of F380 and F382 residues of G3BP1 crucial for SPOCD1-AS/G3BP1 interaction and structure of GIP modeled by Swiss-Model and Pymol. d MeT-5A cells were treated with GIP (15 μmol/l and 30 μmol/l) or control peptides (15 μmol/l and 30 μmol/l) for 48 h. Confocal images of green GIP or control peptides in the cytoplasm. Membrane was stained with red wheat germ agglutinin. Scale bar, 20 μm. e Biotin-labeled peptide pull-down assays with extracted total RNA of MeT-5A cells. The histogram showed enriched SPOCD1-AS expression, calculated as input%. f-i MeT-5A cells were treated with GIP or control peptides for 48 h as mentioned earlier, SPOCD1-AS RNA pull-down assays were conducted with extracted protein of MeT-5A cells using SPOCD1-AS sense probes (f), MMT-related proteins were detected by western blot analysis (g), migration and adhesion assays were performed. Scale bar, 100 μm (h, i). j Ctrl pep and GIP (3 mg/kg) were intraperitoneal injected every other day from week 1 for 10 times (n = 5 for each group). The mice were sacrificed at week 6. Bioluminescence images of the dissected primary tumor and the peritoneal metastatic tumors at the time of killing. Photon count was calculated and showed as median with interquartile range. *p < 0.05. Data are representative of at least three independent experiments and are presented as mean ± SD unless otherwise stated. *p < 0.05, **p < 0.01, ***p < 0.001

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