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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Chemotherapy-elicited exosomal miR-378a-3p and miR-378d promote breast cancer stemness and chemoresistance via the activation of EZH2/STAT3 signaling

Fig. 2

Chemotherapy-elicited breast cancer cell-derived exosomes and their cargo RNAs induce chemoresistance in vivo and in vitro. a Diagram of animal experiments for chemotherapy-induced drug resistance in exosomes. b Images of tumors in mice (n = 20). c Tumor onset and volume. Red arrows indicate the time of exosome treatments, and blue arrows indicate the time of DOX treatments. d, e The effects of DOX and PTX on the cell viability of CAL51 and MDA231 cells co-cultured with chemo-naïve exosomes, DOX chemotherapy-elicited exosomes, PTX chemotherapy-elicited exosomes or chemotherapy-elicited exosomes combined with GW4869. f, g CAL51 and MDA231 cells were cocultured with chemo-naïve exosomes, DOX chemotherapy-elicited exosomes or PTX chemotherapy-elicited exosomes for 48 h and then evaluated using CD44+/CD24- population f and 3D sphere formation g assays. h miR-378a-3p and miR-378d contents in chemo-naïve exosomes and DOX or PTX chemotherapy-elicited exosomes. i Changes in miR-378a-3p and miR-378d levels in CAL51 cells co-cultured with chemo-naïve exosomes and DOX or PTX chemotherapy-elicited exosomes for 48 h. j, k CAL51 and MDA231 cells were transfected with miR-378a-3p or miR-378d mimics or negative control mimics and then exposed to DOX or PTX to evaluate cell viability. l, m At 48 h after transfection with miR-378a-3p or miR-378d mimics, inhibitors and the corresponding normal control, cells were collected for CD44+/CD24- population l and sphere formation m assays. *p < 0.05, ** p < 0.01, *** p < 0.001

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