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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Chemotherapy-elicited exosomal miR-378a-3p and miR-378d promote breast cancer stemness and chemoresistance via the activation of EZH2/STAT3 signaling

Fig. 3

Chemo-elicited exosomal miR-378a-3p and miR-378d regulated the WNT/β-catenin and Notch stemness pathways by targeting NUMB and DKK3. a Analysis of miRNA-378 expression data by Gene set enrichment analysis revealed that genes involved in the WNT and NOTCH pathways were significantly enriched. b MiRanda prediction of the binding sites of miR-378a-3p/miR-378d within the 3′-UTR of DKK3/NUMB. c, d Relative levels of DKK3/NUMB mRNA in CAL51 cells transfected with miR-378a-3p or miR-378d mimics and the corresponding normal control. e–h MiR-378a-3p/miR-378d was found to be directly bound to the NUMB/DKK3 3′-UTR using a dual-luciferase reporter assay. i, j Western blot analysis of the expression of DKK3, β-catenin, Cyclin D1, C-MYC, NUMB, NOTCH1, HES1 and HEY1 in the different groups. i CAL51 and MDA231 cells were transfected with miR-378a-3p or miR-378d mimics, inhibitors and the corresponding normal control for 48 h before western blot analysis. j Western blot results showing the expression levels of the indicated proteins in CAL51 and MDA231 cells at 48 h after simultaneous transfection with the DKK3 or NUMB expression plasmid or empty vector and miR-378a-3p or miR-378d mimics or the corresponding normal control. *p < 0.05, ** p < 0.01, *** p < 0.001

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