Fig. 2

TYK2 knockdown suppresses ESCC growth. a Upper panel: TYK2 protein level in the normal esophagus and ESCC cell lines; lower panel: TYK2 knockdown results in KYSE140 and KYSE450 cells. b Left panel: KYSE140 cell viability; right panel: KYSE450 cell viability. c Left panel: the representative colony images of ESCC cell lines. Scale bar: 200 μm. Middle panel: Statistical analysis of the clone ratio in KYSE140 cells. Right panel: Statistical analysis of the clone ratio in KYSE450 cells. d The tumor pictures of the cell-derived tumor xenograft (CDX) mouse model. e The tumor growth status after transplanted TYK2 knockdown cells in the CDX mouse model. f The analysis of tumor weight of the CDX mouse. Student’s unpaired t-test was used in (b, c, e, and f). Asterisks (*p < 0.05, ***p < 0.001) indicate a significant change