Fig. 2

ATAD2 promotes ESCC cell proliferation in vitro and tumorigenesis in vivo. a, b Knockdown of ATAD2 by three shRNAs and restoration of ATAD2 expression in KYSE30 and KYSE510 cells were confirmed by western blot analysis. The following functional experiments in KYSE30 and KYSE510 cells are all grouped in accordance with (b). c Effect of altered ATAD2 expression on cell growth by CCK8 assay. d Effect of altered ATAD2 expression on cell colony formation and colonies of each group were quantified. e Effect of altered ATAD2 expression in KYSE510 cells on tumor growth in nude mice (n = 7 tumors per group), tumor size was measured every 3 days after 1 week after injection. Tumor tissues were confirmed by hematoxylin-eosin staining. Data was presented as mean ± standard deviation (SD) (*P < 0.05; **P < 0.01; *** P < 0.001)