Fig. 3
ATAD2 promotes ESCC cell migration, invasion in vitro and metastasis in vivo. a, b Effect of altered ATAD2 expression on cell migration and invasion ability using transwell assay. The migrated and invasive cells were counted and analyzed. c Macroscopic appearances of lung images of each group, black arrows indicate the tumor nodules. Lung metastasis nodules were confirmed by hematoxylin-eosin staining. Number of metastatic nodules in each group was counted and analyzed. Data was analyzed using unpaired student’s t-test and presented as mean ± SD (*P < 0.05; **P < 0.01, *** P < 0.001)