Fig. 4

ATAD2 activates TGF-β signaling pathway and TGF-β1 gene expression. a KEGG pathway analysis using significantly differentially expressed genes between ATAD2 knockdown KYSE510 cells and control cells based on RNA-sequencing data. b TGF-β response element luciferase activity induced by ATAD2 in KYSE30 and KYSE 510 cells. c ATAD2 and TGF-β1 expression in a panel of ESCC cell lines analyzed by western blot. Expression of ATAD2 and TGF-β1 protein were quantified and analyzed using Pearson correlation method. d Effect of ATAD2 knockdown and restoration on TGF-β1 analyzed by western blot. e Effect of ATAD2 overexpression on TGF-β1 analyzed by western blot