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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: ATAD2 interacts with C/EBPβ to promote esophageal squamous cell carcinoma metastasis via TGF-β1/Smad3 signaling

Fig. 5

ATAD2 exerts pro-metastatic function depending on the TGF-β signaling induced epithelial-mesenchymal transition. A, B Effect of TGF-β1 receptor inhibition on KYSE510 and KYSE30 cell migration and invasion ability using transwell assay. TGF-β1 receptor was inhibited with 5 μM LY2157299. The migrated and invasive cells were counted and analyzed. C Effect of ATAD2 knockdown on transcription factor Snail, epithelial marker E-cadherin and mesenchymal markers Vimentin and N-cadherin analyzed by western blot. D1-4, E Effect of TGF-β1 receptor inhibition on transcription factor Snail, epithelial marker E-cadherin and mesenchymal markers Vimentin and N-cadherin analyzed by qRT-PCR (D1-4) and western blot (E) respectively. For qRT-PCR, TGF-β1 receptor was inhibited with 2.5 μM, 5 μM, 10 μM LY2157299 and SB525334 for 24 h, and 5 μM LY2157299 and SB525334 were applied for 48 h for western blot analysis. Data was presented as mean ± SD (*P < 0.05; **P < 0.01, *** P < 0.001)

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