Fig. 6
ATAD2 regulates TGF-β1/Smad3 pathway which is independent of Smad2 and Smad4. a Effect of ATAD2 knockdown and restoration on expression of TGF-β1 and its downstream effectors analyzed by western blot in KYSE30 and KYSE510 cells using the whole cell extracts. b Effect of ATAD2 overexpression on TGF-β1 and its downstream effectors analyzed by western blot in TE-1 and EC9706 cells using the whole cell extracts. c, d Effect of ATAD2 knockdown and restoration on TGF-β1 and its downstream effectors analyzed by Western blot in KYSE30 and KYSE510 using cytoplasmic and nuclear proteins separately. e Effect of ATAD2 knockdown and restoration on TGF-β1 and its downstream effectors in xenograft tumors. f-h Effects of ATAD2 knockdown and restoration on localization of p-Smad2 (green) (f), p-Smad3 (green) (g), Smad4 (green) (h) and DAPI (blue) performed by immunofluorescence staining