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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: ATAD2 interacts with C/EBPβ to promote esophageal squamous cell carcinoma metastasis via TGF-β1/Smad3 signaling

Fig. 7

ATAD2 facilitates nuclear translocation of C/EBPβ which directly activates TGF-β1 gene transcription. a Effect of ATAD2 knockdown on TGF-β1 mRNA expression in KYSE30 and KYSE510 cells by qRT-PCR. b TGF-β1 luciferase activity induced by C/EBPβ in KYSE510 and KYSE30 cells. c Schematic figure summarizing ChIP-PCR primer sets in TGF-β1 promoter region. d C/EBPβ directly bound to the TGF-β1 promoter regions (region #2–4, #6–8, and #11–13). e The interaction between ATAD2 and C/EBPβ was verified by co-immunoprecipitation. Anti-Flag antibody was used to immunoprecipitate Flag-tagged C/EBPβ from whole cell extracts prepared from HEK293T cells co-expressing V5-ATAD2 and Flag-C/EBPβ. f Endogenous interaction of ATAD2 and C/EBPβ in KYSE510 cells verified by co-immunoprecipitation. g Effect of ATAD2 knockdown on C/EBPβ expression by western blot in KYSE30 and KYSE510 cells using cytoplasmic and nuclear proteins separately. Data was presented as mean ± SD (*P < 0.05; **P < 0.01)

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