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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Exosomal miR-106b-5p derived from melanoma cell promotes primary melanocytes epithelial-mesenchymal transition through targeting EphA4

Fig. 3

Melanoma cell-secreted exosomal miR-106b-5p promotes the EMT of melanocytes. a Cellular and exosomal miR-106b-5p levels in melanoma cells stably transfected with miR-106b-5p inhibitor or NC lentivectors were detected by qRT-PCR. b miR-106b-5p levels in HEMa-LP cells pre-treated with non-exosomes or melanoma exosomes with different treatment factors. c Western blots identified E-cadherin, N-cadherin, fibronectin and Snail protein expression changes in HEMa-LP cells treated with non-exosomes or indicated exosomes, GAPDH was used as a control. d N-cadherin were identified by immunofluorescence assays in HEMa-LP cells treated with non-exosomes or indicated exosomes. Scale bar, 100 μm. e The invasive capacity of HEMa-LP cells treated with non-exosomes or indicated exosomes was assessed by the transwell assay. Scale bar, 100 μm. f Migration capacity of HEMa-LP cells in different treatment groups was monitored by the scratch wound assay. Scale bar, 200 μm. g The ability of HEMa-LP cells adhesion to fibronectin was detected by the adhesion assay. Scale bar, 100 μm. Data were expressed as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001

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