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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Exosomal miR-106b-5p derived from melanoma cell promotes primary melanocytes epithelial-mesenchymal transition through targeting EphA4

Fig. 5

Exosomal miR-106b-5p promotes the EMT of melanocytes by targeting EphA4 to activate the ERK pathway. a Western blot analysis of EphA4, E-cadherin, N-cadherin, fibronectin and Snail, total ERK and p-ERKT202/Y204 in HEMa-LP treated with indicated exosomes in the presence of EphA4 plasmid or EphA4 siRNA, GAPDH was used as a control. b N-cadherin were identified by immunofluorescence assays in HEMa-LP cells treated with indicated exosomes in the presence of EphA4 plasmid or EphA4 siRNA. Scale bar, 100 μm. c The invasive capacity of HEMa-LP cells treated with indicated exosomes in the presence of EphA4 plasmid or EphA4 siRNA was assessed by the transwell assay. Scale bar, 100 μm. d Migration ability of HEMa-LP cells in different treatment groups was detected by the scratch wound assay. Scale bar, 200 μm. e The ability of HEMa-LP cells in different treatment groups adhesion to fibronectin was detected by the adhesion assay. Scale bar, 100 μm. Data were expressed as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001

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