Fig. 1

Expression of ASS1 is upregulated in HCC spheroids a Schematic of the proteomics analysis of Korean patient-derived HCC cells cultured in monolayers (2D) or spheroids (3D). b Expression of identified proteins including HSPD1, peroxiredoxin, HSF5, Nucleophosmin, ALDOA, and ASS1 in 2D or 3D-cultured HCC. Expression of ASS1 in 2D or 3D-culured system from Fa2N-4 and HCC cell lines by c Western blot assay and d qRT-PCR. Expression of ASS1 in patient-derived HCC cell lines e Western blot and f qRT-PCR. ^**P < 0.01 and ^***P < 0.001 compared to control groups