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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Argininosuccinate synthase 1 suppresses tumor progression through activation of PERK/eIF2α/ATF4/CHOP axis in hepatocellular carcinoma

Fig. 3

Overexpression of ASS1 inhibits HCC tumor growth and improves chemotherapy efficiency a Cell viability analysis of Huh7 and SNU475 and b Doubling time analysis of Huh7, SNU475 and SNU449 cells transfected with pCMV3 or ASS1-flag. The measurement of c Annexin V staining apoptosis analysis, d Caspase3/7 activity and e Expression of cleaved PARP (upper panel) and the quantitative analysis graph (bottom panel) in Huh7 and SNU475 cells transfected with pCMV3 or ASS1-Flag. f Wound healing assays of Huh7 cells transfected with pCMV3 or ASS1-Flag. g Expression of EMT-related proteins, including a-SMA, N-cadherin, E-cadherin, and Snail (left panel) and the quantitative analysis graph (right panel) in Huh7 and SNU475 cells transfected with pCMV3 or ASS1-Flag. Cell survival curves of h siRNA against ASS1-transfected in Hep3B cells and i ASS1 transfected in Huh7 cells treated with cisplatin at the indicated concentrations. *P < 0.05, **P < 0.01 and ***P < 0.001 compared to control group

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