Fig. 2

Effect of IR on MITF expression. a Flow cytometry analysis of MITF and MLANA in different melanoma cell lines and effect of IR (10 Gy, 24 h). Quantitative analysis is showed in Fig. S7. b Time and dosage effect of IR on the expression of MITF and MLANA analyzed by Western blot. In all cases, β-actin was used as a load control. c Effect of IR on MITF expression in IGR39 melanoma cells analyzed by Western blot. d Confocal microscopy analysis of MITF in IGR39 melanoma cells under indicated conditions (IR, 10 Gy). (Bars, 15 μm). *p < 0.05 when compared fluorescence in IR vesrsus non-IR groups. e qRT-PCR analysis of MITF mRNA in indicated melanoma cells before and after IR (10 Gy; 24 h). Relative mRNA expression in IGR39 cells was normalized with respect to untreated IGR37 cells. *p < 0.05. f Confocal microscopy analysis of MITF and MLANA before and after IR (24 h) in siControl and siMITF transfected melanoma cells. (Bars, 15 μm). Histograms represent the fluorescent intensity per cell in different assayed conditions. *p < 0.05 when compared fluorescence in IR versus non-IR groups. Quantification of blots presented in this figure can be visualized in Fig. S8. The groupings blots in this figure were cropped from different gels. Full blots are shown in the Supplementary Information, Fig. S9