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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: NLRP7 deubiquitination by USP10 promotes tumor progression and tumor-associated macrophage polarization in colorectal cancer

Fig. 3

Degradation of NLRP7 is repressed by USP10 deubiquitination. a USP10 upregulation increased NLRP7 protein levels, but (b) had no significant effect on mRNA levels of NLRP7 in CRC cells. c In cycloheximide (CHX) chase, NLRP7 protein stability was decreased in HCT116 cells with knockdown of USP10 compared to control. d USP10 knockdown increased the ubiquitination of NLRP7 in HCT116 cells. e Mapping of NLRP7 using truncation mutants. f Half-life analysis of transfected wild-type and truncation mutant NLRP7-FLAG and (h) transfected K-R point mutant NLRP7-FLAG in CRC cells. Densitometric analysis of (g) NLRP7 truncation mutant and (i) NLRP7 K-R point mutant signal versus time. j Immunopurified NLRP7-FLAG K379 mutant showed decreased ubiquitination compared to wild-type in HCT116 cells with shUSP10. **P < 0.01, and *P < 0.05. NS: no significance

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