Fig. 6

CCL2 secretion from CRC cells is necessary for NLRP7 induction of TAM polarization. a-b The results of qPCR and flow cytometry confirmed that recombinant CCL2 rescued the M2-like macrophage polarization effect from CM of NLRP7 knockdown cells, and CCL2-neutralizing antibody suppressed M2-like macrophage polarization effect from CM of NLRP7 overexpressing cells. c Macrophages in xenograft tumor tissues from control, NLRP7 knockdown, USP10 knockdown, and USP10 knockdown with NLRP7 overexpression (magnification: × 600). Scale bars, 50 μm. d Expression level of TAM functional polarization markers in xenograft tumor tissues. e CD86-positive M1 macrophages and CD163-positive M2 macrophages in xenograft tumor tissues from four groups (magnification: × 600). Scale bars, 50 μm. f The expression levels of CCL2 in peripheral blood and tumor tissues of animals from four groups. ***P < 0.001, **P < 0.01, and *P < 0.05. NS: no significance